GHR和IGF-IR在济宁青山羊子宫内分布和表达的发育性变化

doi:10.11843/j.issn.0366-6964.2013.09.009

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (9): 1392-1399.doi: 10.11843/j.issn.0366-6964.2013.09.009

GHR和IGF-IR在济宁青山羊子宫内分布和表达的发育性变化

同海妮^1,2，白淑^1,2，王树迎^1,2*，黄丽波^1,2，石运芝^1,2，侯衍猛^1,2

（1. 山东农业大学动物科技学院，泰安 271018； 2. 山东省动物生物技术与疫病防治重点实验室，泰安 271018）

收稿日期:2013-04-08 出版日期:2013-09-23 发布日期:2013-09-23
通讯作者: 王树迎，教授，E-mail：sywang@sdau.edu.cn
作者简介:同海妮（1986-），女，陕西韩城人，硕士生，主要从事动物形态学和发育生物学研究，E-mail：tonghainisdau@163.com
基金资助:
国家自然科学基金（30871832）；山东省自然科学基金（ZR2009DM027）；山东省科技攻关项目（2008GG30008018）

Developmental Changes of the Distribution of GHR and IGF-IR and the Expression of Its mRNA in the Uterus of Jining Grey Goats

TONG Hai-ni^1,2, BAI Shu^1,2, WANG Shu-ying^1,2*, HUANG Li-bo^1,2,SHI yun-zhi^1,2, HOU Yan-meng^1,2

(1. College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, China; 2. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai’an 271018, China)

Received:2013-04-08 Online:2013-09-23 Published:2013-09-23

摘要/Abstract

摘要：

旨在探讨济宁青山羊生后发育过程中生长激素受体（Growth hormone，GHR）和胰岛素样生长因子I受体（Insulin-like growth factor-I，IGF-IR）在子宫的分布及其mRNA的表达规律。运用实时荧光定量PCR技术，检测GHR mRNA和IGF-IR mRNA的表达规律；SABC法检测GHR和IGF-IR免疫阳性细胞在子宫的分布。结果表明，GHR和IGF-IR免疫阳性染色主要见于子宫腺管上皮、基质、血管内皮和子宫平滑肌细胞的胞质中，偶见胞核着色。GHR和IGF-IR的阳性细胞数与其mRNA表达的变化趋势基本一致，且IGF-IR低于GHR。 GHR和IGF-IR在初情期（60日龄）和性成熟（120日龄）达到高峰（P<0.01），120日龄后下降，并维持在一个相对稳定的水平。GHR和IGF-IR在子宫的生长发育过程中起着重要作用，且初情期和性成熟期可能是济宁青山羊生后子宫发育最快的时期。

Abstract:

To study the localization of GHR and IGF-IR and the expression of GHR mRNA and IGF-IR mRNA in uterus of Jining Grey goats during postnatal development. Real-time flourescence quantitative PCR was used to detect the expression pattern of GHR mRNA and IGF-IR mRNA, and SABC method was applied to research the distribution of positive cells of GHR and IGF-IR in the uterus. GHR and IGF-IR were mainly localized in the cytoplasm of glandular epithelium, stromal cells, endothelium and smooth muscle cells of the uterus, occasionally in the nuclei. The changed trends on the positive cells of GHR and IGF-IR were similar with that on the expression of its mRNA. Although the expression of IGF-IR was less than that of GHR, the expression of GHR and IGF-IR both reached its peak on the puberty (D60) and the sexual maturity (D120)（P<0.01）, Then decreased and remained at a relatively stable level. GHR and IGF-IR play an important role in the growth of the uterus, and the puberty and the sexual maturity may be the fastest period of the uterus during postnatal development.

中图分类号:

S827
S811.2

同海妮，白淑，王树迎，黄丽波，石运芝，侯衍猛. GHR和IGF-IR在济宁青山羊子宫内分布和表达的发育性变化[J]. 畜牧兽医学报, 2013, 44(9): 1392-1399.

TONG Hai-ni, BAI Shu, WANG Shu-ying, HUANG Li-bo,SHI yun-zhi, HOU Yan-meng. Developmental Changes of the Distribution of GHR and IGF-IR and the Expression of Its mRNA in the Uterus of Jining Grey Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1392-1399.

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GHR和IGF-IR在济宁青山羊子宫内分布和表达的发育性变化

Developmental Changes of the Distribution of GHR and IGF-IR and the Expression of Its mRNA in the Uterus of Jining Grey Goats

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